2
Department of Microbiology, Plant and Microbe Biot, Yerevan State University
Abstract
This study reports the purification and biochemical characterization of thermostable and acidic-pH-stable α-amylase from Bacillus sp. Iranian S1 isolated from the desert soil (Gandom-e-Beryan in Lut desert, Iran). Amylase production was found to be growth associated. Maximum enzyme production was in exponential phase with activity 2.93 U ml-1 at 50°C and pH 5. The enzyme was purified by isopropanol sedimentation, ion-exchange chromatography on DEAE cellulose DE-52 and gel filtration on Sephadex G-100. The enzyme was purified to homogeneity with purification fold 98.9 with 25% yield and specific activity 375.9 U mg-1 of protein. The molecular weight of purified α-amylase was estimated to be 70 KDa by SDS-PAGE. The enzyme remained stable in a wide range of temperature and pH between 30°C- 110°C and 3-9, respectively. Assayed with soluble starch as substrate, the enzyme displayed optimal activity at 90°C and pH 5. The purified α-amylase was acid- and thermo-stable with novel properties making it suitable for many industrial purposes.
Popov,Y. , Terchunyan,A. , Panosyan,H. and sharifi,S. (2013). Purification and Characterization of a Novel Thermostable and Acid Stable α-Amylase from Bacillus Sp. Iranian S1. International Journal of Engineering, 26(8), 815-820.
MLA
Popov,Y. , , Terchunyan,A. , , Panosyan,H. , and sharifi,S. . "Purification and Characterization of a Novel Thermostable and Acid Stable α-Amylase from Bacillus Sp. Iranian S1", International Journal of Engineering, 26, 8, 2013, 815-820.
HARVARD
Popov Y., Terchunyan A., Panosyan H., sharifi S. (2013). 'Purification and Characterization of a Novel Thermostable and Acid Stable α-Amylase from Bacillus Sp. Iranian S1', International Journal of Engineering, 26(8), pp. 815-820.
CHICAGO
Y. Popov, A. Terchunyan, H. Panosyan and S. sharifi, "Purification and Characterization of a Novel Thermostable and Acid Stable α-Amylase from Bacillus Sp. Iranian S1," International Journal of Engineering, 26 8 (2013): 815-820,
VANCOUVER
Popov Y., Terchunyan A., Panosyan H., sharifi S. Purification and Characterization of a Novel Thermostable and Acid Stable α-Amylase from Bacillus Sp. Iranian S1. IJE, 2013; 26(8): 815-820.
