1
Biochemical & Chemical Engineering, Noshirvani University of Technology, Babol
2
Chemical & Petroleum Engineering, University of Sains Malaysia
3
Chemical Engineering, Universiti Sains Malaysia Minden
Abstract
The synthesis of citronellyl butyrate by direct esterification reaction catalyzed by immobilized lipase from Candida rugosa was studied in a continuous packed bed reactor using n-hexane as organic solvent. Parameters such as residence time, temperature, and pH were examined. The optimum conversion was obtained at a flow rate of 1 ml/min (residence time 8 min), temperature of 50 °C, and pH 7.5. At high temperature, biocatalyst was rapidly deactivated with respect to time. The immobilized lipase was stable at pH range of 6 to 9. The deactivation of biocatalyst was increased at pH of immobilized lipase 4 and 10 in comparison with pH range from 5 to 9. The esterification reaction in packed-bed reactor was modeled by ping pong model and ping pong with product inhibition model. The observed kinetics behaviour of esterification reaction was found to follow a ping pong with product inhibition model. In this study, the conversion of butyric acid and stability of immobilized lipase was higher in the system with molecular-sieve than in the system without molecular-sieve.
Najafpour, G., Kamaruddin, A. H., & Dahlan, I. (2005). Citronellyl Butyrate Synthesis in Non-Conventional Media Using Packed-Bed Immobilized Candida Rugosa Lipase Reactor. International Journal of Engineering, 18(2), 153-164.
MLA
Ghasem Najafpour; A. H. Kamaruddin; Irvan Dahlan. "Citronellyl Butyrate Synthesis in Non-Conventional Media Using Packed-Bed Immobilized Candida Rugosa Lipase Reactor". International Journal of Engineering, 18, 2, 2005, 153-164.
HARVARD
Najafpour, G., Kamaruddin, A. H., Dahlan, I. (2005). 'Citronellyl Butyrate Synthesis in Non-Conventional Media Using Packed-Bed Immobilized Candida Rugosa Lipase Reactor', International Journal of Engineering, 18(2), pp. 153-164.
VANCOUVER
Najafpour, G., Kamaruddin, A. H., Dahlan, I. Citronellyl Butyrate Synthesis in Non-Conventional Media Using Packed-Bed Immobilized Candida Rugosa Lipase Reactor. International Journal of Engineering, 2005; 18(2): 153-164.