TY - JOUR ID - 71950 TI - Optimization of Lipase Immobilization JO - International Journal of Engineering JA - IJE LA - en SN - 1025-2495 AU - Alemzadeh, Iran AU - Sayyar Kavardi, Seyyedeh Sepideh AU - Kazemi, A. AD - Chemical Engineering, Sharif University of Technology AD - Department of Chemical and Petroleum Engineering, Sharif University of Technology AD - Biochemal eng., sharif university Y1 - 2012 PY - 2012 VL - 25 IS - 1 SP - 1 EP - 10 KW - Pseudomonas aeruginosa KW - Lipase KW - production KW - Purification KW - Immobilization KW - Calcium Alginate DO - N2 - Pseudomonas aeruginosa BBRC-10036 was used for lipase production. The organism secreted the enzyme extracellulary. In order to purify the enzyme, precipitation was done first, and then this lipase has been purified by Ion exchange Chromatography leading to 2.3-fold purification and 11.47% recovery. Lipase from P.aeruginosa was entrapped into Ca-alginate gel beads and effect of independent variables such as alginate concentration (%w/v), CaCl2 concentration (M) and enzyme load (%v/v) on immobilization yield and activity of immobilized enzyme were investigated. Media optimization for immobilization of lipase was carried out by Response Surface Methodology. The optimum conditions were as follows: sodium alginate concentration 2.5% (w/v), calcium chloride concentration 2.5(M) and enzyme load 50% (v/v). At those conditions, the highest immobilization yield and the optimum activity of immobilized enzyme, respectively, obtained were 93.65% and 2.64 unit/gr(IME). UR - https://www.ije.ir/article_71950.html L1 - https://www.ije.ir/article_71950_12403f28b643200ea7eb4e3eba695a0f.pdf ER -