TY - JOUR ID - 106022 TI - Immobilization of Laccase from Trametes hirsuta onto CMC Coated Magnetic Nanoparticles JO - International Journal of Engineering JA - IJE LA - en SN - 1025-2495 AU - Sadeghzadeh, S. AU - Ghazvini, S. AU - Hejazi, S. AU - Yaghmaei, S. AU - Ghobadi Nejad, Z. AD - Chemical & Petroleum Engineering Department, Sharif University of Technology, Tehran, Iran AD - Biochemical & Bioenvironmental Research Center, Sharif University of Technology, Azadi Avenue, Tehran, Iran Y1 - 2020 PY - 2020 VL - 33 IS - 4 SP - 513 EP - 519 KW - Laccase KW - Immobilization KW - Fe3O4/CMC KW - Trametes hirsuta DO - 10.5829/ije.2020.33.04a.01 N2 - In this study, Fe3O4/CMC magnetic nanoparticles were synthesized through co-precipitation method. Afterward, laccase from Trametes hirsuta was immobilized onto Carboxymethyl cellulose (CMC)-coated magnetic Fe3O4 nanoparticles by covalent bonding between carboxyl groups of carboxymethyl cellulose and amine group of laccases. Also, the resulted magnetic nanoparticles and immobilized laccase were characterized by Fourier-transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) and dynamic light scattering (DLS) analysis. Moreover, the vital factors in enzyme immobilization, such as contact time, amount of N-hydroxysuccinimide (NHS), and the amount of nanoparticles were optimized, which successively 48 h, 0.01 g, and 0.0125 g were achieved for 0.01g of N-ethyl-N′-(3-dimethylaminopropyl)carbodiimide (EDC). Activity recovery of 51 ± 0.8% was achieved by optimizing the immobilization process. The results also indicated that the loading of laccase onto carboxymethyl cellulose-coated Fe3O4 nanoparticles was approximately 120 (mg/g). Finally, the immobilized laccases on magnetic support could save nearly 50% of their initial activity after five consecutive cycles.   UR - https://www.ije.ir/article_106022.html L1 - https://www.ije.ir/article_106022_684a8f217b2f636d5cbd3157c4ea4bcf.pdf ER -