The suitability of hemp fibres as green support material for immobilization of Acidithiobacillus ferrooxidans cells in ferrous iron bio-oxidation process was investigated. A series of experiments were carried out in shake flasks and bioreactor for both cell immobilization and iron bio-oxidation. In flask experiments, five successive batch colonization cycles resulted in 6-fold increase in ferrous iron bio-oxidation rate. Evaluation of immobilized cells activity through two approaches as wet and dry storage demonstrated the higher activity of wet stored bacteria than dry ones after a period of two months. The dry stored bacteria lost almost all their activities after eight weeks. Whereas, the activity of wet stored samples was 30% at the same time. In bioreactor experiments, use of a packed-bed bioreactor with hemp fibres, the maximum ferrous iron bio-oxidation rate of 2.46 kg m-3 h-1 was obtained at dilution rate of 3.97 h-1, and retention time of 0.25 h. Our experimental results could pave a new platform for the application of hemp fibre as an inexpensive and environmentally friendly support material in bacterial cell immobilization, especially in ferrous iron bio-oxidation process using acidophilic bacteria.
Akhlaghi, N. (2019). Hemp Fibres as Novel Green Support Material for Immobilization of Acidithiobacillus ferrooxidans. International Journal of Engineering, 32(9), 1225-1230. doi: 10.5829/ije.2019.32.09c.01
MLA
Neda Akhlaghi. "Hemp Fibres as Novel Green Support Material for Immobilization of Acidithiobacillus ferrooxidans". International Journal of Engineering, 32, 9, 2019, 1225-1230. doi: 10.5829/ije.2019.32.09c.01
HARVARD
Akhlaghi, N. (2019). 'Hemp Fibres as Novel Green Support Material for Immobilization of Acidithiobacillus ferrooxidans', International Journal of Engineering, 32(9), pp. 1225-1230. doi: 10.5829/ije.2019.32.09c.01
VANCOUVER
Akhlaghi, N. Hemp Fibres as Novel Green Support Material for Immobilization of Acidithiobacillus ferrooxidans. International Journal of Engineering, 2019; 32(9): 1225-1230. doi: 10.5829/ije.2019.32.09c.01