Process Performance of a Granular Single Bioreactor with Continuous Feeding and Intermittent Discharge Regime Treating Dairy Wastewater

Document Type: Original Article


Environmental research Center (ERC), Department of Applied Chemistry, Faculty of Chemistry, Razi University, Kermanshah, Iran


In this study, granulated sludge synthesized in a sequence batch reactor (SBR) and then granulated sludge was transferred to continuous feeding and intermittent discharge (CFID) bioreactor. Two independent variables (air flow rate and hydraulic retention time (HRT)) were considered to optimize the process. Subsequently, long term performance (150 days) of the bioreactor was assessed by monitoring nine responses. The process optimization in CFID regime was performed using a central composite face-centered design (CCFD) and analyzed using response surface methodology (RSM). Based on obtained data, the optimum conditions were achieved at HRT of 7-8 h and air flow rate of 2-3.5 L/min. The CFID with granular sludge acted as a high rate bioreactor as the granular sludge could provide high MLSS concentrations (around 10000 mg/L)  in a bioreactor.


1.     Su, Y., Mennerich, A., and Urban, B., “Coupled nutrient removal and biomass production with mixed algal culture: Impact of biotic and abiotic factors”, Bioresource Technology,  Vol. 118, (2012), 469–476.

2.     Latif, M.A., Ghufran, R., Wahid, Z.A., and Ahmad, A., “Integrated application of upflow anaerobic sludge blanket reactor for the treatment of wastewaters”, Water Research,  Vol. 45, No. 16, (2011), 4683–4699.

3.     Omil, F., Garrido, J.M., Arrojo, B., and Méndez, R., “Anaerobic filter reactor performance for the treatment of complex dairy wastewater at industrial scale”, Water Research,  Vol. 37, No. 17, (2003), 4099–4108.

4.     Tawfik, A., Sobhey, M., and Badawy, M., “Treatment of a combined dairy and domestic wastewater in an up-flow anaerobic sludge blanket (UASB) reactor followed by activated sludge (AS system)”, Desalination,  Vol. 227, No. 1–3, (2008), 167–177.

5.     Adav, S.S., Lee, D.J., and Lai, J.Y., “Proteolytic activity in stored aerobic granular sludge and structural integrity”, Bioresource Technology,  Vol. 100, No. 1, (2009), 68–73.

6.     Wei, Y., Ji, M., Li, R., and Qin, F., “Organic and nitrogen removal from landfill leachate in aerobic granular sludge sequencing batch reactors”, Waste Management,  Vol. 32, No. 3, (2012), 448–455.

7.     Wang, S.-G., Liu, X.-W., Gong, W.-X., Gao, B.-Y., Zhang, D.-H., and Yu, H.-Q., “Aerobic granulation with brewery wastewater in a sequencing batch reactor”, Bioresource Technology,  Vol. 98, No. 11, (2007), 2142–2147.

8.     Cassidy, D.P., and Belia, E., “Nitrogen and phosphorus removal from an abattoir wastewater in a SBR with aerobic granular sludge”, Water Research,  Vol. 39, No. 19, (2005), 4817–4823.

9.     Figueroa, M.., Mosquera-Corral, A.., Campos, J.L.., and Méndez, R., “Treatment of saline wastewater in SBR aerobic granular reactors”, Water Science & Technology,  Vol. 58, No. 2, (2008), 479–485.

10.   Abdullah, N., Ujang, Z., and Yahya, A., “Aerobic granular sludge formation for high strength agro-based wastewater treatment”, Bioresource Technology,  Vol. 102, No. 12, (2011), 6778–6781.

11.   Schwarzenbeck, N., Borges, J.M., and Wilderer, P.A., “Treatment of dairy effluents in an aerobic granular sludge sequencing batch reactor”, Applied Microbiology and Biotechnology,  Vol. 66, No. 6, (2005), 711–718.

12.   Arrojo, B., Mosquera-Corral, A., Garrido, J.M., and Méndez, R., “Aerobic granulation with industrial wastewater in sequencing batch reactors”, Water Research,  Vol. 38, No. 14–15, (2004), 3389–3399.

13.   Li, X.-M., Guo, L., Yang, Q., Zeng, G.-M., and Liao, D.-X., “Removal of carbon and nutrients from low strength domestic wastewater by expanded granular sludge bed-zeolite bed filtration (EGSB-ZBF) integrated treatment concept”, Process Biochemistry,  Vol. 42, No. 8, (2007), 1173–1179.

14.   Liu, Y., and Liu, Q.S., “Causes and control of filamentous growth in aerobic granular sludge sequencing batch reactors”, Biotechnology Advances,  Vol. 24, No. 1, (2006), 115–127.

15.   Zheng, Y.-M., Yu, H.-Q., Liu, S.-J., and Liu, X.-Z., “Formation and instability of aerobic granules under high organic loading conditions”, Chemosphere,  Vol. 63, No. 10, (2006), 1791–1800.

16.   Chen, X., Simon, E.S., Xiang, Y., Kachman, M., Andrews, P.C., and Wang, Y., “Quantitative proteomics analysis of cell cycle-regulated Golgi disassembly and reassembly.”, The Journal of Biological Chemistry,  Vol. 285, No. 10, (2010), 7197–7207.

17.   Asadi, A., Zinatizadeh, A.A.L., Sumathi, S., Rezaie, N., and Kiani, S., “A Comparative Study on Performance of Two Aerobic Sequencing Batch Reactors with Flocculated and Granulated Sludge Treating an Industrial Estate Wastewater: Process Analysis and Modeling”, International Journal of Engineering - Transactions B: Applications,  Vol. 26, No. 2, (2012), 105–116.

18.   Rezaee, S., Zinatizadeh, A.A.L., and Asadi, A., “Comparative study on effect of mechanical mixing and ultrasound on the performance of a single up-flow anaerobic/aerobic/anoxic bioreactor removing CNP from milk processing wastewater”, Journal of the Taiwan Institute of Chemical Engineers,  Vol. 58, (2016), 297–309.

19.   Rezaee, S., Zinatizadeh, A.A.L., and Asadi, A., “High rate CNP removal from a milk processing wastewater in a single ultrasound augmented up-flow anaerobic/aerobic/anoxic bioreactor”, Ultrasonics Sonochemistry,  Vol. 23, (2015), 289–301.

20.   Wünsch, B., Heine, W., and Neis, U., “Combatting bulking sludge with ultrasound”, TU Hamburg-Harburg Reports on Sanitary Engineering,  Vol. 35, (2002), 201–212.

21.   Asadi, A., Zinatizadeh, A.A., and Van Loosdrecht, M., “High rate simultaneous nutrients removal in a single air lift bioreactor with continuous feed and intermittent discharge regime: Process optimization and effect of feed characteristics”, Chemical Engineering Journal,  Vol. 301, (2016), 200–209.

22.   Asadi, A., Zinatizadeh, A.A., and Van Loosdrecht, M., “A novel continuous feed and intermittent discharge airlift bioreactor (CFIDAB) for enhanced simultaneous removal of carbon and nutrients from soft drink industrial wastewater”, Chemical Engineering Journal,  Vol. 292, (2016), 13–27.

23.   Khuri, A., and Cornell, J., Response surfaces: designs and analyses, Marcel Dekker, New York, (1996).

24.   APHA, Standard methods for the examination of water and wastewater, American Public Health Association, and American Water Works Association, (2005).

25.   Lateef, A., Chaudhry, M.N., and Ilyas, S., “Biological treatment of dairy wastewater using activated sludge”, ScienceAsia,  Vol. 39, (2013), 179–185.

26.   Arrojo, B., Figueroa, M., Mosquera-Corral, A., Campos, J.L., and Méndez, R., “Influence of gas flow-induced shear stress on the operation of the Anammox process in a SBR”, Chemosphere,  Vol. 72, No. 11, (2008), 1687–1693.

27.   Liu, Y., and Tay, J.-H., “State of the art of biogranulation technology for wastewater treatment”, Biotechnology Advances,  Vol. 22, No. 7, (2004), 533–563.

28.   Mosquera-Corral, A., de Kreuk, M.K., Heijnen, J.J., and van Loosdrecht, M.C.M., “Effects of oxygen concentration on N-removal in an aerobic granular sludge reactor”, Water Research,  Vol. 39, No. 12, (2005), 2676–2686.

29.   Liu, Y.-Q., and Tay, J.-H., “Characteristics and stability of aerobic granules cultivated with different starvation time”, Applied Microbiology and Biotechnology,  Vol. 75, No. 1, (2007), 205–210.

30.   Amini, M., Younesi, H., Zinatizadeh Lorestani, A.A., and Najafpour, G., “Determination of optimum conditions for dairy wastewater treatment in UAASB reactor for removal of nutrients”, Bioresource Technology,  Vol. 145, (2013), 71–79.

31.   Coats, E.R., Mockos, A., and Loge, F.J., “Post-anoxic denitrification driven by PHA and glycogen within enhanced biological phosphorus removal”, Bioresource Technology,  Vol. 102, No. 2, (2011), 1019–1027.

32.   Bamforth, S.M., and Singleton, I., “Bioremediation of polycyclic aromatic hydrocarbons: current knowledge and future directions”, Journal of Chemical Technology & Biotechnology,  Vol. 80, No. 7, (2005), 723–736.

33. Asadi, A., Zinatizadeh, A.A.L., and Hasnain Isa, M., “Performance of intermittently aerated up-flow sludge bed reactor and sequencing batch reactor treating industrial estate wastewater: A comparative study”, Bioresource Technology,  Vol. 123, (2012), 495–506.

34.   De Kreuk, M.K., Heijnen, J.J., and van Loosdrecht, M.C.M., “Simultaneous COD, nitrogen, and phosphate removal by aerobic granular sludge”, Biotechnology and Bioengineering,  Vol. 90, No. 6, (2005), 761–769.

35.   Broughton, A., Pratt, S., and Shilton, A., “Enhanced biological phosphorus removal for high-strength wastewater with a low rbCOD:P ratio”, Bioresource Technology,  Vol. 99, No. 5, (2008), 1236–1241.

36.   Arrojo, B., Mosquera-Corral, A., Campos, J.L., and Méndez, R., “Effects of mechanical stress on Anammox granules in a sequencing batch reactor (SBR)”, Journal of Biotechnology,  Vol. 123, No. 4, (2006), 453–463.