Separation of Curcumin from Curcuma longa L. and its Conjugation with Silica Nanoparticles for Anti-cancer Activities

Authors

1 Biotechnology Research Laboratory, Faculty of Chemical Engineering, Babol Noshirvani University of Technology, Babol, Iran

2 Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran

Abstract

Curcumin is the natural bio-active component of turmeric (Curcuma longa L.) with known therapeutic properties; nevertheless, its biological applications are limited due to its poor bioavailability. To overcome this limitation, curcumin was conjugated with silica nanoparticles. Curcumin was separated from turmeric by microwave-assisted extraction and silica nanoparticles were developed from rice husk. Conjugation of curcumin with silica nanoparticles was performed through ultrasound-assisted wet impregnation. XRD, FTIR and UV-visible analyses confirmed the successful synthesis of the conjugate; the drug loading in the nanoparticle was 39% as determined by HPLC analysis. TEM and AFM analyses indicated the spherical morphology of the conjugate with average particle size of 85.9 nm. The cell killing activity of the conjugate was tested against HeLa, MCF-7 and Saos-2 cancer cell lines and normal fibroblast cell line using MTT assay. The silica:curcumin conjugate was effective for destruction of cancerous cells, especially HeLa cells, with minimum side effects on healthy fibroblasts.

Keywords

References

1.   Nasri, H., Shahinfard, N., Rafieian, M., Rafieian, S., Shirzad, M. and Rafieian, M., "Turmeric: A spice with multifunctional medicinal properties", Journal of Herbmed Pharmacology,Vol. 3, No. 1, (2014), 5-8.
2.   Singh, S.P., Sharma, M. and Gupta, P.K., "Cytotoxicity of curcumin silica nanoparticle complexes conjugated with hyaluronic acid on colon cancer cells", International Journal of Biological Macromolecules,Vol. 74, No. (2015), 162-170.
3.   Dey, S. and Sreenivasan, K., "Conjugation of curcumin onto alginate enhances aqueous solubility and stability of curcumin", Carbohydrate Polymers,Vol. 99, No. (2014), 499-507.
4.   Athinarayanan, J., Periasamy, V.S., Alhazmi, M., Alatiah, K.A. and Alshatwi, A.A., "Synthesis of biogenic silica nanoparticles from rice husks for biomedical applications", Ceramics International,Vol. 41, No. 1, (2015), 275-281.
5.   Alshatwi, A.A., Athinarayanan, J. and Periasamy, V.S., "Biocompatibility assessment of rice husk-derived biogenic silica nanoparticles for biomedical applications", Materials Science and Engineering C,Vol. 47, No. (2015), 8-16.
6. Carvalho DdM, Takeuchi KP, Geraldine RM, Moura CJd, Torres MCL. Production, solubility and antioxidant activity of curcumin nanosuspension. Journal of Food Science and Technology, 35: 115-9 (2015).
7.   Tang, L. and Cheng, J., "Nonporous silica nanoparticles for nanomedicine application", Nano Today,Vol. 8, No. 3, (2013), 290-312.
8.   Bitar, A., Ahmad, N.M., Fessi, H. and Elaissari, A., "Silica-based nanoparticles for biomedical applications", Drug Discovery Today,Vol. 17, No. 19, (2012), 1147-1154.
9.   Liou, T.-H. and Yang, C.-C., "Synthesis and surface characteristics of nanosilica produced from alkali-extracted rice husk ash", Materials Science and Engineering: B, Vol. 176, No. 7, (2011), 521-529.
10. Gangwar, R.K., Tomar, G.B., Dhumale, V.A., Zinjarde, S., Sharma, R.B. and Datar, S., "Curcumin conjugated silica nanoparticles for improving bioavailability and its anticancer applications", Journal of Agricultural and Food Chemistry,Vol. 61, No. 40, (2013), 9632-9637.
11. Singh, S.P., Sharma, M. and Gupta, P.K., "Enhancement of phototoxicity of curcumin in human oral cancer cells using silica nanoparticles as delivery vehicle", Lasers in Medical Science.,Vol. 29, No. 2, (2014), 645-652.
12. Rezaei, S., Najafpour, G., Mohammadi, M., Moghadamnia, A., Kazemi, S. and Bengmark, S., "Formic Acid and Microwave Assisted Extraction of Curcumin from Turmeric (Curcuma longa L.)", International Journal of Engineering,Vol. 29, No. (2016), 145-151.
13. Sahne, F., Mohammadi, M., Najafpour, G.D. and Moghadamnia, A.A., "Enzyme-assisted ionic liquid extraction of bioactive compound from turmeric (Curcuma longa L.): Isolation, purification and analysis of curcumin", Industrial Crops and Products,Vol. 95, No. (2017), 686-694.
14. Hu, K., Huang, X., Gao, Y., Huang, X., Xiao, H. and McClements, D.J., "Core–shell biopolymer nanoparticle delivery systems: Synthesis and characterization of curcumin fortified zein–pectin nanoparticles", Food Chemistry,Vol. 182, No. (2015), 275-281.
15. Mathew, A., Fukuda, T., Nagaoka, Y., Hasumura, T., Morimoto, H., Yoshida, Y., Maekawa, T., Venugopal, K. and Kumar, D.S., "Curcumin loaded-PLGA nanoparticles conjugated with Tet-1 peptide for potential use in Alzheimer's disease", PLoS One,Vol. 7, No. 3, (2012), e32616.
16. Mosmann, T., "Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays", Journal of Immunological Methods,Vol. 65, No. 1-2, (1983), 55-63.
17. Dey, S. and Sreenivasan, K., "Conjugating curcumin to water soluble polymer stabilized gold nanoparticles via pH responsive succinate linker", Journal of Materials Chemistry B,Vol. 3, No. 5, (2015), 824-833.
18. Bettini, S., Pagano, R., Valli, L. and Giancane, G., "Drastic nickel ion removal from aqueous solution by curcumin-capped Ag nanoparticles", Nanoscale,Vol. 6, No. 17, (2014), 10113-10117.
19. Shweta, K. and Jha, H., "Rice husk extracted lignin–TEOS biocomposites: Effects of acetylation and silane surface treatments for application in nickel removal", Biotechnology Reports,Vol. 7, No. (2015), 95-106.
20. Masuda, T., Hidaka, K., Shinohara, A., Maekawa, T., Takeda, Y. and Yamaguchi, H., "Chemical studies on antioxidant mechanism of curcuminoid: analysis of radical reaction products from curcumin", Journal of Agricultural and Food Chemistry,Vol. 47, No. 1, (1999), 71-77.
21. Lv, L., Shen, Y., Liu, J., Wang, F., Li, M., Li, M., Guo, A., Wang, Y., Zhou, D. and Guo, S., "Enhancing curcumin anticancer efficacy through di-block copolymer micelle encapsulation", Journal of Biomedical Nanotechnology,Vol. 10, No. 2, (2014), 179-193.
22. Avnesh S. Thakor and Gambhir, S.S., "Nanooncology: The Future of Cancer Diagnosis and Therapy", CA CANCER J CLIN,Vol. 63, No. (2013), 395-418.
23. Davis, M.E. and Shin, D.M., "Nanoparticle therapeutics: an emerging treatment modality for cancer", Nature Reviews Drug Discovery,Vol. 7, No. 9, (2008), 771-782.
24. Peer, D., Karp, J.M., Hong, S., Farokhzad, O.C., Margalit, R. and Langer, R., "Nanocarriers as an emerging platform for cancer therapy", Nature Nanotechnology,Vol. 2, No. 12, (2007), 751-760.
International Journal of Engineering
Volume 31, Issue 11
TRANSACTIONS B: Applications
November 2018
Pages 1803-1809

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