Analysis of Bacterial Contaminant in Pasir Gudang, Johor Tap Water Supply–Varies pH Value Observation


Department of Control and Mechatronics Engineering, School of Electrical Engineering, Universiti Teknologi Malaysia, Skudai, Johor, Malaysia


The number of breakthrough pathogenic activity in water distribution network system is constantly increasing day by day especially at level of consumption. Bacterial growth or survival rate often relates to acidity and alkalinity of water. Sudden changes in pH value and temperature indicates a possibility of present bacterial contaminant in aqueous environment. The observation of pH- and temperature-based for tap water supply samples in Pasir Gudang regions therefore was determined. On the basis of the findings, the observed pH value was compared to the recommended range for pH tap and drinking water, which is between 6.5-8.5. A significant spread can be seen among the measured parameters within the range of pH and temperature at 6.00 to 8.65 and 19.20 to 32.00 °C, respectively. There is a statistically significant difference between each sampling regions based on the measured pH value (  44.79,  determined by one-way ANOVA. The pH value and temperature evidence a significant effects by the location of tap water samples near industrial regions. There also appears to be a negative Pearson correlation between the two water parameters in four out of five regions.


1.     NAM, E.F.V., "Quantifying water and energy linkages in irrigation",  (2017).

2.     Outlook, A.W.D., "Measuring water security in asia and the pacific", manila,  (2013).

3.     Richardson, S.D., "Water analysis: Emerging contaminants and current issues", Analytical chemistry,  Vol. 81, No. 12, (2009), 4645-4677.

4.     Ashbolt, N.J., "Microbial contamination of drinking water and human health from community water systems", Current environmental health reports,  Vol. 2, No. 1, (2015), 95-106.

5.     Asghari, F.B., Jaafari, J., Yousefi, M., Mohammadi, A.A. and Dehghanzadeh, R., "Evaluation of water corrosion, scaling extent and heterotrophic plate count bacteria in asbestos and polyethylene pipes in drinking water distribution system", Human and Ecological Risk Assessment: An International Journal,  Vol. 24, No. 4, (2018), 1138-1149.

6.     Varughese, E.A., Brinkman, N.E., Anneken, E.M., Cashdollar, J.L., Fout, G.S., Furlong, E.T., Kolpin, D.W., Glassmeyer, S.T. and Keely, S.P., "Estimating virus occurrence using bayesian modeling in multiple drinking water systems of the united states", Science of the Total Environment,  Vol. 619, (2018), 1330-1339.

7.     Lehtola, M.J., Miettinen, I.T. and Martikainen, P.J., "Microbiological quality control in distribution systems", Water Encyclopedia,  Vol. 2, No., (2005), 243-247.

8.     Ikonen, J., Pitkänen, T., Kosse, P., Ciszek, R., Kolehmainen, M. and Miettinen, I.T., "On-line detection of escherichia coli intrusion in a pilot-scale drinking water distribution system", Journal of environmental management,  Vol. 198, (2017), 384-392.

9.     EBRAHIMI, A., Asadi, M. and NAJAFPOUR, G.D., "Dairy wastewater treatment using three-stage rotating biological contactor (nrbc)",  (2009).

10.   Kotlarz, N., Rockey, N., Olson, T.M., Haig, S.-J., Sanford, L., LiPuma, J.J. and Raskin, L., "Biofilms in full-scale drinking water ozone contactors contribute viable bacteria to ozonated water", Environmental science & technology,  Vol. 52, No. 5, (2018), 2618-2628.

11.   Juhna, T., Birzniece, D., Larsson, S., Zulenkovs, D., Sharipo, A., Azevedo, N., Menard-Szczebara, F., Castagnet, S., Feliers, C. and Keevil, C., "Detection of escherichia coli in biofilms from pipe samples and coupons in drinking water distribution networks", Applied and environmental microbiology,  Vol. 73, No. 22, (2007), 7456-7464.

12.   Aziz, R.K., Khalifa, M.M. and Sharaf, R.R., "Contaminated water as a source of helicobacter pylori infection: A review", Journal of advanced research,  Vol. 6, No. 4, (2015), 539-547.

13.   Kim, U., Ravikumar, A., Seubert, J. and Figueira, S., "Detection of bacterial pathogens through microfluidic DNA sensors and mobile interface toward rapid, affordable, and point-of-care water monitoring", in Point-of-Care Healthcare Technologies (PHT), IEEE, (2013), 1-4.

14.   Ramírez-Castillo, F.Y., Loera-Muro, A., Jacques, M., Garneau, P., Avelar-González, F.J., Harel, J. and Guerrero-Barrera, A.L., "Waterborne pathogens: Detection methods and challenges", Pathogens,  Vol. 4, No. 2, (2015), 307-334.

15.   Maurer, L.M., Yohannes, E., Bondurant, S.S., Radmacher, M. and Slonczewski, J.L., "Ph regulates genes for flagellar motility, catabolism, and oxidative stress in escherichia coli k-12", Journal of bacteriology,  Vol. 187, No. 1, (2005), 304-319.

16.   Vanhauteghem, D., Janssens, G.P.J., Lauwaerts, A., Sys, S., Boyen, F., Cox, E. and Meyer, E., "Exposure to the proton scavenger glycine under alkaline conditions induces escherichia coli viability loss", PLoS one,  Vol. 8, No. 3, (2013), 1-11.

17.   Padan, E., Bibi, E., Ito, M. and Krulwich, T.A., "Alkaline ph homeostasis in bacteria: New insights", Biochimica et biophysica acta (BBA)-biomembranes,  Vol. 1717, No. 2, (2005), 67-88.

18.   Zhao, T., Doyle, M. and Besser, R., "Fate of enterohemorrhagic escherichia coli o157: H7 in apple cider with and without preservatives", Applied and environmental microbiology,  Vol. 59, No. 8, (1993), 2526-2530.

19.   Najafpour, G., Azizan, A. and Harun, A., "Microbial desulfurization of malaysian coal in batch process using mixed culture", IJE Transactions B: Applications,  Vol. 15, No. 3, (2001), 227-234.

20.   Agency, E.P., "Parameters of water quality: Interpretation and standards", Dublin, Ireland, (2001).

21.   Council, P.G.M., "Cousilator of majlis perbandaran pasir gudang", Pasir Gudang Municipal Council, (2017).

22.   Agency, E.P., "Quick guide to drinking water sample collection", United States, (2005).

23.   Association, A.P.H. and Association, A.W.W., "Standard methods for the examination of water and wastewater", American public health association,  (1989).

24.   World Health Organization, "Guidelines for drinking-water quality", World Health Organization, Fourth Edition,  (2011).

25.   World Health Organization, "In guidelines for drinking-water quality: Surveillance and control of community supplies, Geneva, World Health Organization Vol. 3,  (1997).

26.   F. Sacher and B. Hambsch, "State-of-the-art in drinking water monitoring", TECHNEAU: Safe Drinking Water from Source to Tap,  (2009), 135-143.

27.   World Health Organization, "Guidelines for drinking-water quality: Ph in drinking-water", World Health Organization (2007).

 28.   Payment, P., Waite, M. and Dufour, A., "Introducing parameters for the assessment of drinking water quality", Assessing microbial safety of drinking water,  Vol. 4, (2003), 47-77.

29.   Ohno, A., Kato, N., Yamada, K. and Yamaguchi, K., "Factors influencing survival of legionella pneumophila serotype 1 in hot spring water and tap water", Applied and environmental microbiology,  Vol. 69, No. 5, (2003), 2540-2547.

30.   Miller, H.C., Morgan, M.J., Wylie, J.T., Kaksonen, A.H., Sutton, D., Braun, K. and Puzon, G.J., "Elimination of naegleria fowleri from bulk water and biofilm in an operational drinking water distribution system", Water research,  Vol. 110, (2017), 15-26.

31.   Fish, K., Osborn, A. and Boxall, J., "Biofilm structures (eps and bacterial communities) in drinking water distribution systems are conditioned by hydraulics and influence discolouration", Science of the Total Environment,  Vol. 593, (2017), 571-580.

32.   Leščešen, I., Pantelić, M., Dolinaj, D., Stojanović, V. and Milošević, D., "Statistical analysis of water quality parameters of the drina river (west serbia), 2004-11", Polish Journal of Environmental Studies,  Vol. 24, No. 2, (2015), 555-561.

33.   Bhat, S.A., Meraj, G., Yaseen, S. and Pandit, A.K., "Statistical assessment of water quality parameters for pollution source identification in sukhnag stream: An inflow stream of lake wular (ramsar site), kashmir himalaya", Journal of Ecosystems,  Vol. 2014, (2014).

34.   Darren, G. and Mallery, P., "Spss for windows step by step: A simple guide and reference", 10th Editi. Pearson, Boston, (2010).

35.   Gravetter, F.J. and Wallnau, L.B., "Essentials of statistics for the behavioral sciences (psy 200 (300) quantitative methods in psychology), Boston: Cengage Learning,  (2010).

36.   USEPA, "Online source water quality monitoring for water quality surveillance and response systems", U.S.Environmental Protection Agency, (2016).

37.   Agency, E.P., "Ground water contamination", Environmental Protection Agency, (2015).

38.   Agency, E.P., "Source water protection practices bulletin. Managing stormwater runoff to prevent contamination of drinking water", Washington, DC, USA, (2009).

39.   World Health Organization, "Microbial fact sheets", World health organization guidelines for drinking-water quality (WHO GDWQ),  (2011), 271-273.

40.   McDade, J.E., "Legionella and the prevention of legionellosis", Emerg. Infect. Dis,  Vol. 14, No., (2008), 1006.

41.   Cabral, J.P., "Water microbiology. Bacterial pathogens and water", International journal of environmental research and public health,  Vol. 7, No. 10, (2010), 3657-3703.

42.   Maghsoodi, V. and Ghobadi, Z., "Biological pretreatment of a beverage waste using yeast isolated from the factory sludge", International Journal of Engineering Transaction B: Applications,  Vol. 15, No. 3, (2002), 223-226.

43.   Evans, J.D., "Straightforward statistics for the behavioral sciences", Brooks/Cole Publishing Company  (1996).