Inhibitory Effects of Swietenia Mahagoni Seeds Extract on Α-Glucosidase and Α-amylase

Authors

1 Center of Lipids Engineering and Applied Research (CLEAR), Ibnu Sina Institute Scientific & Industrial Research (Ibnu Sina ISIR), Universiti Teknologi Malaysia, Johor, Malaysia

2 Department of Bioprocess and Polymer Engineering, School of Chemical and Energy Engineering, University Teknologi Malaysia, Johor, Malaysia

3 Advanced Membrane Technology Research Centre (AMTEC), Universiti Teknologi Malaysia, Skudai, Johor, Malaysia

4 Natural Product Division, Forest Research Institute Malaysia (FRIM), Selangor Darul Ehsan, Malaysia

Abstract

This study analyzed the inhibition activity of Swietenia mahagoni seeds extract on α-glucosidase and α-amylase enzymes inhibition assays.  Swietenia mahagoni seeds were extracted by using Supercritical Carbon Dioxide (SC-CO2) extraction at pressures of 20- 30 MPa and temperatures of 40- 60°C.  The oil yields obtained were analyzed with α- glucosidase and α- amylase enzymes inhibition assays.  All data obtained were expressed as mean ± standard deviation for triplicate experiments.  One way analysis was used for statistical significance by using Statistica software version 7.0 (StartSoft, EUA) and IC50 (extract concentration causing 50% enzyme inhibitory) was determined by using GraphPad Prism 6.0 software.  Swietenia mahagoni seeds extract have a strong inhibition of α-glucosidase enzyme activity (98.4% ± 0.2) but a moderate inhibition of α- amylase enzyme activity (34.9% ± 1.2). These findings implied that Swietenia mahagoni seeds extract could be an effective natural antidiabetic agent.

Keywords


1.     Adefegha, S.A., Oboh, G., Adefegha, O.M., Boligon, A.A. and Athayde, M.L., "Antihyperglycemic, hypolipidemic, hepatoprotective and antioxidative effects of dietary clove (szyzgium aromaticum) bud powder in a high‐fat diet/streptozotocin‐induced diabetes rat model", Journal of the Science of Food and Agriculture,  Vol. 94, No. 13, (2014), 2726-2737.

2.     Kumar, S., Narwal, S., Kumar, V. and Prakash, O., "Α-glucosidase inhibitors from plants: A natural approach to treat diabetes", Pharmacognosy Reviews,  Vol. 5, No. 9, (2011), 19.

3.     Association, A.D., "Diagnosis and classification of diabetes mellitus", Diabetes Care,  Vol. 37, No. Supplement 1, (2014), S81-S90.

4.     Cheng, A.Y. and Fantus, I.G., "Oral antihyperglycemic therapy for type 2 diabetes mellitus", Canadian Medical Association Journal,  Vol. 172, No. 2, (2005), 213-226.

5.     Casirola, D.M. and Ferraris, R.P., "Α-glucosidase inhibitors prevent diet-induced increases in intestinal sugar transport in diabetic mice", Metabolism,  Vol. 55, No. 6, (2006), 832-841.

6.     WRESDIYATI, T., SA'DIAH, S., Winarto, A. and Febriyani, V., "Alpha-glucosidase inhibition and hypoglycemic activities of sweitenia mahagoni seed extract", HAYATI Journal of Biosciences,  Vol. 22, No. 2, (2015), 73-78.

7.     Tas, S., Sarandol, E., Ziyanok, S., Aslan, K. and Dirican, M., "Effects of green tea on serum paraoxonase/arylesterase activities in streptozotocin-induced diabetic rats", Nutrition research,  Vol. 25, No. 12, (2005), 1061-1074.

8.     Triggle, C.R. and Ding, H., "Cardiovascular impact of drugs used in the treatment of diabetes", Therapeutic advances in chronic disease,  Vol. 5, No. 6, (2014), 245-268.

9.     Goh, B.H., Abdul Kadir, H., Abdul Malek, S. and Ng, S.W., "Swietenolide diacetate from the seeds of swietenia macrophylla", Acta Crystallographica Section E: Structure Reports Online,  Vol. 66, No. 6, (2010), o1396-o1396.

10.   Goh, B.H. and Kadir, H.A., "In vitro cytotoxic potential of swietenia macrophylla king seeds against human carcinoma cell lines", Journal of Medicinal Plants Research,  Vol. 5, No. 8, (2011), 1395-1404.

11.   Maiti, A., Dewanjee, S., Kundu, M. and Mandal, S.C., "Evaluation of antidiabetic activity of the seeds of swietenia macrophylla in diabetic rats", Pharmaceutical Biology,  Vol. 47, No. 2, (2009), 132-136.

12.   Balijepalli, M.K., Suppaiah, V., Chin, A.-m., Buru, A.S., Sagineedu, S.R. and Pichika, M.R., "Acute oral toxicity studies of swietenia macrophylla seeds in sprague dawley rats", Pharmacognosy Research,  Vol. 7, No. 1, (2015), 38.

13.   Liza, M., Rahman, R.A., Mandana, B., Jinap, S., Rahmat, A., Zaidul, I. and Hamid, A., "Supercritical carbon dioxide extraction of bioactive flavonoid from strobilanthes crispus (pecah kaca)", Food and Bioproducts Processing,  Vol. 88, No. 2-3, (2010), 319-326.

14.   Liza, M.S., Russly, A.R., Jinap, S., Azizah, H. and Md, Z., "Optimization of extraction condition for supercritical carbon dioxide (scco 2) extraction of strobhilantes crispus (pecah kaca) leaves by response surface methodology", Journal of Food Processing and Technology,  Vol. 4, No. 1, (2013).

15.   Foster, N., Mammucari, R., Dehghani, F., Barrett, A., Bezanehtak, K., Coen, E., Combes, G., Meure, L., Ng, A. and Regtop, H.L., "Processing pharmaceutical compounds using dense gas technology", Industrial & Engineering Chemistry Research,  Vol. 42, No. 25, (2003), 6476-6493.

16.   Schuler, I., Milon, A., Nakatani, Y., Ourisson, G., Albrecht, A.-M., Benveniste, P. and Hartman, M.-A., "Differential effects of plant sterols on water permeability and on acyl chain ordering of soybean phosphatidylcholine bilayers", Proceedings of the National Academy of Sciences,  Vol. 88, No. 16, (1991), 6926-6930.

17.   Saeidnia, S., Manayi, A., Gohari, A.R. and Abdollahi, M., "The story of beta-sitosterol-a review", European Journal of Medicinal Plants,  Vol. 4, No. 5, (2014), 590.

18.   Loizou, S., Lekakis, I., Chrousos, G.P. and Moutsatsou, P., "Β‐sitosterol exhibits anti‐inflammatory activity in human aortic endothelial cells", Molecular Nutrition & Food Research,  Vol. 54, No. 4, (2010), 551-558.

19.   Ju, Y.H., Clausen, L.M., Allred, K.F., Almada, A.L. and Helferich, W.G., "Β-sitosterol, β-sitosterol glucoside, and a mixture of β-sitosterol and β-sitosterol glucoside modulate the growth of estrogen-responsive breast cancer cells in vitro and in ovariectomized athymic mice", The Journal of Nutrition,  Vol. 134, No. 5, (2004), 1145-1151.

20.   Miettinen, T.A. and Gylling, H., "Ineffective decrease of serum cholesterol by simvastatin in a subgroup of hypercholesterolemic coronary patients", Atherosclerosis,  Vol. 164, No. 1, (2002), 147-152.

21.   Vivancos, M. and Moreno, J.J., "Β-sitosterol modulates antioxidant enzyme response in raw 264.7 macrophages", Free Radical Biology and Medicine,  Vol. 39, No. 1, (2005), 91-97.

22.   Gupta, R., Sharma, A.K., Dobhal, M., Sharma, M. and Gupta, R., "Antidiabetic and antioxidant potential of β‐sitosterol in streptozotocin‐induced experimental hyperglycemia", Journal of Diabetes,  Vol. 3, No. 1, (2011), 29-37.

23.   Maiti, A., Dewanjee, S. and Sahu, R., "Isolation of hypoglycemic phytoconstituent from swietenia macrophylla seeds", Phytotherapy Research,  Vol. 23, No. 12, (2009), 1731-1733.

24.   Hashim, M.A., Yam, M.F., Hor, S.Y., Lim, C.P.P., Asmawi, M.Z. and Sadikun, A., "Anti-hyperglycaemic activity of swietenia macrophylla king (meliaceae) seed extracts in normoglycaemic rats undergoing glucose tolerance tests", Chinese Medicine,  Vol. 8, No. 1, (2013), 11.

25.   Hajra, S., Mehta, A., Pandey, P., John, J., Mehta, P. and Vyas, S., "Free radical scavenging and α-amylase inhibitory activity of swietenia mahagoni seeds oil", International Journal of Pharmacognosy and Phytochemical Research,  Vol. 5, No. 1, (2013), 51-56.

26.   Karan, S.K., Mishra, S.K., Pal, D. and Mondal, A., "Isolation of-sitosterol and evaluation of antidiabetic activity of aristolochia indica in alloxan-induced diabetic mice with a reference to in-vitro antioxidant activity", Journal of Medicinal Plants Research,  Vol. 6, No. 7, (2012), 1219-1223.

27.   Sánchez‐Machado, D., López‐Hernández, J., Paseiro‐Losada, P. and López‐Cervantes, J., "An hplc method for the quantification of sterols in edible seaweeds", Biomedical Chromatography,  Vol. 18, No. 3, (2004), 183-190.

28.   Jemain, M.M., Musa'adah, M.N., Rohaya, A., Rashid, L.A. and Hadiani, I.N., "In vitro antihyperglycaemic effects of some malaysian plants", Journal of Tropical Forest Science,  Vol. 23, No. 4, (2011), 467-472.

29.   Bothon, F.T., Debiton, E., Avlessi, F., Forestier, C., Teulade, J.-C. and Sohounhloue, D.K., "In vitro biological effects of two anti-diabetic medicinal plants used in benin as folk medicine", BMC Complementary and Alternative Medicine,  Vol. 13, No. 1, (2013), 51-58.

30.   Rivera-Chávez, J., González-Andrade, M., Del Carmen González, M., Glenn, A. E., and Mata, R., , "A-glucosidase inhibitors from mexu 27095, an endophytic fungus from hintonia latiflora", Phytochemistry,  Vol. 94, (2013), 198-205.

31.   Miller, G.L., "Use of dinitrosalicylic acid reagent for determination of reducing sugar", Analytical Chemistry,  Vol. 31, No. 3, (1959), 426-428.

32.   Ali, H., Houghton, P. and Soumyanath, A., "Α-amylase inhibitory activity of some malaysian plants used to treat diabetes; with particular reference to phyllanthus amarus", Journal of Ethnopharmacology,  Vol. 107, No. 3, (2006), 449-455.

33.   Machmudah, S., Kawahito, Y., Sasaki, M. and Goto, M., "Supercritical co2 extraction of rosehip seed oil: Fatty acids composition and process optimization", The Journal of supercritical fluids,  Vol. 41, No. 3, (2007), 421-428.

34.   Pereira, C.G. and Meireles, M.A.A., "Supercritical fluid extraction of bioactive compounds: Fundamentals, applications and economic perspectives", Food and Bioprocess Technology,  Vol. 3, No. 3, (2010), 340-372.

35.   Hartati Salleh, L., Yunus, A. and Aziz, A., "Optimization of supercritical co2 extraction of swietenia mahagoni seed by response surface methodology", Journal of Teknology and Sciences Engineering,  Vol. 67, No. 1, (2014), 15-20.

36.   Sajfrtová, M., Ličková, I., Wimmerová, M., Sovová, H. and Wimmer, Z., "Β-sitosterol: Supercritical carbon dioxide extraction from sea buckthorn (hippophae rhamnoides l.) seeds", International Journal of Molecular Sciences,  Vol. 11, No. 4, (2010), 1842-1850.

37.   Vilegas, J.H., de Marchi, E. and Lancas, F.M., "Extraction of low‐polarity compounds (with emphasis on coumarin and kaurenoic acid) from mikania glomerata (‘guaco’) leaves", Phytochemical Analysis: An International Journal of Plant Chemical and Biochemical Techniques,  Vol. 8, No. 5, (1997), 266-270.

38.   Kawahito, Y., Kondo, M., Machmudah, S., Sibano, K., Sasaki, M. and Goto, M., "Supercritical co2 extraction of biological active compounds from loquat seed", Separation and Purification Technology,  Vol. 61, No. 2, (2008), 130-135.

 39.   Catchpole, O., Perry, N., Da Silva, B., Grey, J. and Smallfield, B., "Supercritical extraction of herbs i: Saw palmetto, st john's wort, kava root, and echinacea", The Journal of Supercritical Fluids,  Vol. 22, No. 2, (2002), 129-138.

40.   Simandi, B., Kristo, S.T., Kery, A., Selmeczi, L., Kmecz, I. and Kemeny, S., "Supercritical fluid extraction of dandelion leaves", The Journal of Supercritical Fluids,  Vol. 23, No. 2, (2002), 135-142.

41.   András, C.D., Simándi, B., Örsi, F., Lambrou, C., Missopolinou‐Tatala, D., Panayiotou, C., Domokos, J. and Doleschall, F., "Supercritical carbon dioxide extraction of okra (hibiscus esculentus l) seeds", Journal of the Science of Food and Agriculture,  Vol. 85, No. 8, (2005), 1415-1419.

42.   Cossuta, D., Simándi, B., Vági, E., Hohmann, J., Prechl, A., Lemberkovics, É., Kéry, Á. and Keve, T., "Supercritical fluid extraction of vitex agnus castus fruit", The Journal of Supercritical Fluids,  Vol. 47, No. 2, (2008), 188-194.

43.   Díaz-Reinoso, B., Moure, A., Domínguez, H. and Parajó, J.C., "Supercritical co2 extraction and purification of compounds with antioxidant activity", Journal of Agricultural and Food Chemistry,  Vol. 54, No. 7, (2006), 2441-2469.

44.   Kajaria, D., Ranjana, J.T., Tripathi, Y.B. and Tiwari, S., "In-vitro α amylase and glycosidase inhibitory effect of ethanolic extract of antiasthmatic drug—shirishadi", Journal of Advanced Pharmaceutical Technology & Research,  Vol. 4, No. 4, (2013), 206.

45.   Safamansouri, H., Nikan, M., Amin, G., Sarkhail, P., Gohari, A.R., Kurepaz-Mahmoodabadi, M. and Saeidnia, S., "Α-amylase inhibitory activity of some traditionally used medicinal species of labiatae", Journal of Diabetes & Metabolic Disorders,  Vol. 13, No. 1, (2014), 114.