Chemical Eng., BNUT
Biochemical & Chemical Engineering, Noshirvani University of Technology, Babol
In this study, solid-state fermentation of two types of agricultural residues/products for lipase production in a tray-bioreactor was investigated. Rhizopus oryzae was used as a potential fungus strain and two types of agricultural residues including soybean meal and sugarcane bagasse were utilized as substrate. Fermentation was carried out in two different operational conditions: one with controlled temperature and humidity and the other without any controlling unit. Lipase activity remarkably increased in the former system using either of substrates; while maximum lipase activities were achieved after 72 hours of fermentation. Also, optimum conditions for lipase activity were identified with cabin temperature of 35 and 45°C, moisture content of 70 and 80%, pH value of 7.0 and 8.0 and water bath temperature of equally 50°C for bagasse and soybean meal, respectively. Maximum lipase activities under optimum conditions were 199.66 and 235.79U/gds for bagasse and soybean meal, respectively.