TY - JOUR ID - 82474 TI - Influence of Different Culture Selection Methods on Polyhydroxyalkanoate Production at Short-term Biomass Enrichment JO - International Journal of Engineering JA - IJE LA - en SN - 1025-2495 AU - Ahmadi, F. AU - Zinatizadeh, A. A. AU - Asadi, A. AU - Younesi, H. AD - Environmental Research Center (ERC), Department of Applied Chemistry, Faculty of Chemistry, Razi University, Kermanshah, Iran. AD - Environmental research Center (ERC), Department of Applied Chemistry, Faculty of Chemistry, Razi University, Kermanshah, Iran AD - Department of Gas and Petroleum, Yasouj University, Gachsaran, Iran. AD - Department of Environmental science, Faculty of Natural resources and Marine Science, Tarbiat Modares University, Noor, Iran. Y1 - 2019 PY - 2019 VL - 32 IS - 2 SP - 184 EP - 192 KW - Polyhydroxyalkanoate KW - Short-term enrichment time (STE) KW - Acetate KW - Soft-drink industrial wastewater DO - N2 - In this study, the potential of four different culture selection methods under short-term enrichment time (STE) to accumulate PHA-producing bacteria in mixed activated sludge was compared and the most efficient culture selection method was introduced. This means, PHA-producing microbial community was firstly enriched in a sequencing batch bioreactor (SBR) with four different selection methods including an anaerobic-aerobic process (SBR1), a fully aerobic batch process (SBR2), an uncoupled carbon and nitrogen feeding regime (SBR3) and aerobic/anoxic process (SBR4). In the next step, cellular PHA content was maximized in a fed-batch accumulator. From the obtained results, PHA could be accumulated up to 13.2, 10.8, 22.36, and 6 % (mg-PHA/mg-TSS) in SBR1, SBR2, SBR3, and SBR4, respectively. Uncoupled carbon and nitrogen feeding regime (SBR3) showed the best PHA accumulating ability when acetate was used as feed. Also, the SBR3 was fed by soft-drink industrial wastewater to evaluate the capability of the selected strategy for treating real wastewater, which 13.75% of mg-PHA/mg-TSS was achieved. UR - https://www.ije.ir/article_82474.html L1 - https://www.ije.ir/article_82474_6b761294fcf74528f8bd280f6d7b6695.pdf ER -