TY - JOUR ID - 71582 TI - Citronellyl Butyrate Synthesis in Non-Conventional Media Using Packed-Bed Immobilized Candida Rugosa Lipase Reactor JO - International Journal of Engineering JA - IJE LA - en SN - 1025-2495 AU - Najafpour, Ghasem AU - Kamaruddin, A. H. AU - Dahlan, Irvan AD - Biochemical & Chemical Engineering, Noshirvani University of Technology, Babol AD - Chemical & Petroleum Engineering, University of Sains Malaysia AD - Chemical Engineering, Universiti Sains Malaysia Minden Y1 - 2005 PY - 2005 VL - 18 IS - 2 SP - 153 EP - 164 KW - Citronellyl Butyrate KW - Flavor KW - Esterification KW - Immobilized lipase KW - Non KW - Conventional Media KW - Packed KW - Bed Reactor KW - Ping Pong Model DO - N2 - The synthesis of citronellyl butyrate by direct esterification reaction catalyzed by immobilized lipase from Candida rugosa was studied in a continuous packed bed reactor using n-hexane as organic solvent. Parameters such as residence time, temperature, and pH were examined. The optimum conversion was obtained at a flow rate of 1 ml/min (residence time 8 min), temperature of 50 °C, and pH 7.5. At high temperature, biocatalyst was rapidly deactivated with respect to time. The immobilized lipase was stable at pH range of 6 to 9. The deactivation of biocatalyst was increased at pH of immobilized lipase 4 and 10 in comparison with pH range from 5 to 9. The esterification reaction in packed-bed reactor was modeled by ping pong model and ping pong with product inhibition model. The observed kinetics behaviour of esterification reaction was found to follow a ping pong with product inhibition model. In this study, the conversion of butyric acid and stability of immobilized lipase was higher in the system with molecular-sieve than in the system without molecular-sieve. UR - https://www.ije.ir/article_71582.html L1 - https://www.ije.ir/article_71582_19542bd53c3183b40d8c48e19d9ca9c1.pdf ER -